Involvement of Circadian Clock Gene BMAL1 in Doxorubicin-Induced Inflammation in Vascular Smooth Muscle Cells

The molecular clock component Brain and Muscle Arnt-Like protein-1 (BMAL-1) affects various biologic processes, including cell survival, in numerous types. We previously demonstrated that BMAL1 positively regulates proliferation Vascular Smooth Cells (VSMCs). However, its role VSMC inflammation remains unelucidated. Because doxorubicin causes phlebitis associated with vascular inflammation, the present study used cultured VSMCs to investigate whether affected doxorubicin-induced inflammation. Doxorubicin treatment led Increased Interleukin (IL)-6 mRNA expression an increase VSMCs. knockdown significantly increased IL-6 further enhanced also decreased viability of other genes, Per1 Clock. Furthermore, levels nuclear factor erythroid 2-related 2, which has anti-inflammatory effects, knockdown. Finally, NADPH oxidase 4 mRNA, p38? p38? levels, leading total p38 Mitogen-Activated Protein Kinase (MAPK) phosphorylated MAPK. induction caused by was inhibited following pretreatment SB203580, a MAPK inhibitor. Our findings via activation. Moreover, when were low. Thus, may be novel therapeutic target treat inflammatory disease, phlebitis.